Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

2.7.1.74

Official Name

deoxycytidine kinase

Name from literature

deoxycytidine kinase

Pathway from literature

metabolism of gemcitabin

Pathway from KEGG

Nucleotide Metabolism; Purine metabolism; map00230

Nucleotide Metabolism; Pyrimidine metabolism; map00240

Organisms

Human (9606)

Genome localization

4q13.3-q21.1[1633 ],

Comments

Cytosine arabinoside can act as acceptor; all natural nucleoside triphosphates (except dCTP) can act as donors.

Rate-limiting Description

"In addition to inhibition of DNA polymerases and DNA synthesis, clofarabine acts as a strong inhibitor of ribonucleotide reductase (RnR), an enzyme involved in regulating intracellular deoxynucleotide pools, and has a high affinity to the enzyme deoxycytidine kinase (dCyd), the rate-limiting step in nucleoside phosphorylation.A review of the English literature was performed that included original articles and related reviews from the MEDLINE (PubMed) data base and from abstracts based on the publication of meeting materials.Although it was synthesized early in the 1980s, the development of clofarabine was stalled until 1993, when, through efforts at The University of Texas M." (15803490)

"Deficiency in deoxycytidine kinase (dCK), the rate-limiting activating enzyme, has been reported in a number of in vitro models as well as in various clinical situations." (16463058)

"Clofarabine is phosphorylated intracellularly by human deoxycytidine kinase (dCK) to the 5'-monophosphate, which is the rate-limiting step in activation of the prodrug." (16421443)

"The rate-limiting enzyme in this pathway is deoxycytidine kinase (dCK)." (12054682)

"The rate-limiting step in the formation of triphosphate is conversion of F-ara-A to its monophosphate, which is catalyzed by deoxycytidine kinase." (11888330)

Regulatory Information

Upstream transcription factor

6667;7391

Regulatory type

Detail

key enzyme;

"Compelling evidence suggests that deoxycytidine kinase (dCK), a key enzyme in the salvage of deoxyribonucleosides and in the activation of clinically relevant nucleoside analogues, can be regulated by reversible phosphorylation." (17065079)

transcriptional factor;"Sp1(6667),USF(7391)"

"human deoxycytidine kinase promoter activity is regulated by USF and Sp1" (14514691)

phosphorylation;

"deoxycytidine kinase activity is regulated by reversible phosphorylation" (15571259)

phosphorylation;

P27707

phosphorylation;

P27707:from_uniprot:11_Phosphoserine

phosphorylation;

P27707:from_uniprot:74_Phosphoserine

Gene ontology

Gene ontology

GO:0006220 (P) pyrimidine nucleotide metabolic process [P27707 ];
GO:0005634 (C) nucleus [P27707 ];
GO:0016773 (F) phosphotransferase activity, alcohol group ... [P27707 ];
GO:0005829 (C) cytosol [P27707 ];
GO:0005524 (F) ATP binding [P27707 ];
GO:0004137 (F) deoxycytidine kinase activity [P27707 ];

Subcellular localization

Localization

nucleus [P27707 ];

Links

SwissProt

P27707

Entrez Gene

1633

HPRD

00507



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  Last Modified: 2009-03-24  
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