Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

6.2.1.7

Official Name

cholate---CoA ligase

Name from literature

microsomal bile acid coA synthetase

Pathway from literature

cytosolic amidation

Pathway from KEGG

Lipid Metabolism; Bile acid biosynthesis; map00120

Cellular Processes; Endocrine System; PPAR signaling pathway; map03320

Organisms

Human (9606)

Genome localization

19q13.43[10998 ],

Comments

Requires Mg2+ for activity. This membrane-bound enzyme catalyses the first step in the conjugation of bile acids with amino acids, converting bile acids into their acyl-CoA thioesters. The second step involves EC 2.3.1.65, bile acid-CoA:amino acid N-acyltransferase and converts the acyl-CoA thioester into the corresponding N-acyl amidate by conjugation with glycine or taurine [5]. Chenodeoxycholate, deoxycholate, lithocholate and trihydroxycoprostanoate can also act as substrates [6].

Rate-limiting Description

"The K(m) was higher for glycine than taurine both in cytosol and the peroxisomal fraction.These results show that the peroxisomal de novo synthesis of bile acids is rate limiting for peroxisomal amidation, and the microsomal bile acid-CoA synthetase is rate limiting for the cytosolic amidation." (10884298)

Regulatory Information

Upstream transcription factor

3172

Regulatory type

Detail

transcriptional factor;HNF4alpha(3172)

"Mice lacking hepatic HNF4alpha expression exhibited markedly decreased expression of the very long chain acyl-CoA synthase-related gene (VLACSR), a mouse candidate for bile acid-CoA ligase, and BAT. " (14583614)

Gene ontology

Gene ontology

GO:0016021 (C) integral to membrane [Q9Y2P5 ];
GO:0000166 (F) nucleotide binding [Q9Y2P5 ];
GO:0000038 (P) very-long-chain fatty acid metabolic process [Q9Y2P5 ];
GO:0047747 (F) cholate-CoA ligase activity [Q9Y2P5 ];
GO:0005789 (C) endoplasmic reticulum membrane [Q9Y2P5 ];
GO:0004467 (F) long-chain-fatty-acid-CoA ligase activity [Q9Y2P5 ];

Tissue expression

Tissue From HPRD

Testis [04499 ];
Liver [04499 ];

Tissue specificity

Predominantly expressed in liver [Q9Y2P5 ];

Subcellular localization

Localization

endoplasmic reticulum [Q9Y2P5 ];

Links

SwissProt

Q9Y2P5

Entrez Gene

10998

HPRD

04499



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  Last Modified: 2009-03-24  
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