Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

5.3.4.1

Official Name

protein disulfide-isomerase

Name from literature

protein disulfide isomerase

Pathway from literature

protein folding

Pathway from KEGG

Human Diseases; Infectious Diseases; Vibrio cholerae infection; map05110

Cellular Processes; Immune System; Antigen processing and presentation; map04612

Organisms

Human (9606)

Genome localization

16p13.3[64714 ], 3q21.1[10954 ], 17q25[5034 ], 7q35[9601 ], 18q22[54495 ], 15q15[2923 ], 2p25.1[10130 ],

Comments

Needs reducing agents or partly reduced enzyme; the reaction depends on sulfhydryl-disulfide interchange.

Rate-limiting Description

"An examination of the pH-dependence and nature of the rate limiting step for the peptide thiol-disulfide oxidase activity of these enzymes reveals that, with the exception of PDIr, they are all remarkably similar." (16677074)

Regulatory Information

Upstream transcription factor

6774;4831;1999

Regulatory type

Detail

phosphorylation;

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." (15302935#17081983#16964243)

transcriptional factor;redox-sensitive transcription factors

"Accordingly, ERp57 appears to cooperate with Ref-1 in the regulation of gene expression mediated by redox-sensitive transcription factors and in the adaptive response of the cell to oxidative insult." (16962936)

phosphorylation;

P07237

phosphorylation;

P30101

phosphorylation;

Q14554

phosphorylation;

Q15084

phosphorylation;

Q96JJ7

phosphorylation;

Q15084:from_uniprot:428_Phosphoserine

phosphorylation;

Q96JJ7:from_uniprot:268_Phosphoserine

transcriptional factor;STAT3(6774)

"Association of the chaperone glucose-regulated protein 58 (GRP58/ER-60/ERp57) with Stat3 in cytosol and plasma membrane complexes." (12060494)

transcriptional factor;STAT3(6774)

"Association of the chaperone glucose-regulated protein 58 (GRP58/ER-60/ERp57) with Stat3 in cytosol and plasma membrane complexes." (12060494#16189514)

interact with TF;NME2(4831)

"Large-scale mapping of human protein-protein interactions by mass spectrometry." (17353931)

interact with TF;ELF3(1999)

"Large-scale mapping of human protein-protein interactions by mass spectrometry." (17353931)

Gene ontology

Gene ontology

GO:0005788 (C) endoplasmic reticulum lumen [Q15084, Q14554, P30101, P13667, Q13087, P07237 ];
GO:0016491 (F) oxidoreductase activity [Q14554 ];
GO:0008632 (P) apoptotic program [Q13087 ];
GO:0006606 (P) protein import into nucleus [P30101 ];
GO:0005509 (F) calcium ion binding [Q96JJ7, P13667 ];
GO:0006457 (P) protein folding [Q15084, Q14554, Q13087 ];
GO:0006621 (P) protein retention in ER lumen [P30101, Q13087 ];
GO:0009986 (C) cell surface [P07237 ];
GO:0016021 (C) integral to membrane [Q96JJ7 ];
GO:0004656 (F) procollagen-proline 4-dioxygenase activity [P07237 ];
GO:0004629 (F) phospholipase C activity [P30101 ];
GO:0004197 (F) cysteine-type endopeptidase activity [P30101 ];
GO:0009306 (P) protein secretion [P13667 ];
GO:0045454 (P) cell redox homeostasis [Q96JJ7, Q15084, Q14554, P30101, P13667, Q13087, P07237 ];
GO:0042470 (C) melanosome [Q15084, P30101, P13667, P07237 ];
GO:0005515 (F) protein binding [Q15084, P30101, P13667, Q13087, P07237 ];
GO:0005783 (C) endoplasmic reticulum [Q15084, Q14554, P30101, P13667, Q13087, P07237 ];
GO:0006950 (P) response to stress [Q14554 ];
GO:0001666 (P) response to hypoxia [Q13087 ];
GO:0003756 (F) protein disulfide isomerase activity [Q96JJ7, Q15084, Q14554, P30101, P13667, Q13087, P07237 ];
GO:0005886 (C) plasma membrane [P07237 ];
GO:0007165 (P) signal transduction [P30101 ];
GO:0005789 (C) endoplasmic reticulum membrane [Q96JJ7 ];
GO:0005576 (C) extracellular region [P07237 ];
GO:0018401 (P) peptidyl-proline hydroxylation to 4-hydroxy... [P07237 ];
GO:0055114 (P) oxidation reduction [Q14554 ];
GO:0005793 (C) ER-Golgi intermediate compartment [Q15084, P07237 ];

Tissue expression

Tissue From HPRD

Serum [07504 ];
Hippocampus [15591 ];
Substantia nigra [15591 ];
Corpus callosum [15591 ];
Spinal cord [15591 ];
Fetus [15591 ];
Caudate nucleus [15591 ];
Testis [15591 ];
Islets of Langerhans [07504, 07181, 03625 ];
Skeletal muscle [15591, 03625 ];
Liver [15591, 07181, 03625 ];
Brain [08334, 15591, 03625 ];
Spleen [15591 ];
Kidney [08334, 15591, 03625 ];
Thalamus [15591 ];
Neutrophil [06501 ];
Lung [08334, 15591, 03625 ];
Heart [15591, 03625 ];
Pancreas [15591, 03625, 07617 ];
Ovary [07504, 15591, 07181, 03625 ];
Amygdala [15591 ];
Placenta [07504, 08334, 07181, 03625 ];
Cerebellum [15591 ];
Subthalamic nucleus [15591 ];

Tissue specificity

Highly expressed in pancreas [Q13087 ];

Widely expressed. Expressed in brain, testis, lung, skin, kidney, uterus, bone, stomach, liver, prostate, placenta, eye and muscle [Q96JJ7 ];

Subcellular localization

Localization

extracellular matrix [P07237 ];

extracellular [P07237 ];

endoplasmic reticulum [Q96JJ7, Q15084, Q14554, P30101, P13667, Q13087, P07237 ];

membrane [P07237 ];

cytoplasm [Q15084, P30101, P13667, P07237 ];

Links

SwissProt

B3KQU2; B3KWF3; P07237; P13667; P30101; Q13087; Q14554; Q15084; Q96JJ7

Entrez Gene

10130; 10954; 2923; 5034; 54495; 64714; 9601

HPRD

15591; 07181; 07617; 03625; 06501; 08334; 07504



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min