Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

1.1.1.49

Official Name

glucose-6-phosphate dehydrogenase

Name from literature

glucose-6-phosphate dehydrogenase

Pathway from literature

pentose phosphate pathway (PPP)/the GSH- and NADPH-dependent H(2)O(2) elimination by PC12 cells/NADPH production

Pathway from KEGG

Metabolism of Other Amino Acids; Glutathione metabolism; map00480

Carbohydrate Metabolism; Pentose phosphate pathway; map00030

Organisms

Human (9606)

Genome localization

Xq28[2539 ],

Comments

Also acts slowly on beta-D-glucose and other sugars. Certain preparations reduce NAD+ as well as NADP+.

Rate-limiting Description

"Furthermore, the activity and transcript level of the lipogenic enzyme glucose-6-phosphate dehydrogenase, the rate-limiting enzyme of the pentose phosphate pathway, were also about 2-fold higher than that of the wild-type; these data corroborate the flux analysis results." (18029214)

"The principal intracellular reductant NADPH is mainly produced by the pentose phosphate pathway by glucose-6-phosphate dehydrogenase (G6PDH), the rate-limiting enzyme, and by 6-phosphogluconate dehydrogenase." (10329961)

Regulatory Information

Upstream transcription factor

1385

Regulatory type

Detail

key enzyme;

"Glucose-6-phosphate dehydrogenase (G6PD), the key enzyme of the hexose-monophosphate shunt pathway, is an essential enzyme involved in both cell growth and cholesterol metabolism, raising the question as to whether G6PD deficiency may have metabolic and growth implications in a deficient population." (12027950)

phosphorylation;

"Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules." (15951569#15592455)

regulatory enzyme;

"We review here some recent data about glucose-6-phosphate dehydrogenase (G6PD), the first and key regulatory enzyme of the pentose phosphate pathway." (8760336)

transcriptional factor;CREB(1385)

"We have shown two distinct CREB-responsive sites in the glucose-6-phosphatase gene promoter that are responding to a constitutively active CREB or elevated concentrations of the catalytic subunit of cAMP-dependent protein kinase in the nucleus." (15659240)

phosphorylation;

P11413

phosphorylation;

P11413:from_uniprot:401_Phosphotyrosine

phosphorylation;

P11413:from_uniprot:503_Phosphotyrosine

phosphorylation;

P11413:from_uniprot:507_Phosphotyrosine

Gene ontology

Gene ontology

GO:0005536 (F) glucose binding [P11413 ];
GO:0034599 (P) cellular response to oxidative stress [P11413 ];
GO:0005829 (C) cytosol [P11413 ];
GO:0005813 (C) centrosome [P11413 ];
GO:0006010 (P) glucose 6-phosphate utilization [P11413 ];
GO:0046390 (P) ribose phosphate biosynthetic process [P11413 ];
GO:0031399 (P) regulation of protein modification process [P11413 ];
GO:0005488 (F) binding [Q2VF42, Q2Q9B7, A8K8D9, Q2Q9H2, Q0PHS1, A2IBT6 ];
GO:0050661 (F) NADP binding [P11413 ];
GO:0004345 (F) glucose-6-phosphate dehydrogenase activity [Q2VF42, Q2Q9B7, A8K8D9, Q2Q9H2, Q0PHS1, A2IBT6, P11413 ];
GO:0006006 (P) glucose metabolic process [Q2VF42, Q2Q9B7, A8K8D9, Q2Q9H2, Q0PHS1, A2IBT6 ];
GO:0042803 (F) protein homodimerization activity [P11413 ];
GO:0043231 (C) intracellular membrane-bounded organelle [P11413 ];
GO:0006695 (P) cholesterol biosynthetic process [P11413 ];
GO:0009898 (C) internal side of plasma membrane [P11413 ];
GO:0043249 (P) erythrocyte maturation [P11413 ];
GO:0006749 (P) glutathione metabolic process [P11413 ];
GO:0055114 (P) oxidation reduction [Q2VF42, Q2Q9B7, A8K8D9, Q2Q9H2, Q0PHS1, A2IBT6, P11413 ];
GO:0009051 (P) pentose-phosphate shunt, oxidative branch [P11413 ];

Tissue expression

Tissue From HPRD

Hematopoietic stem cell [02377 ];
Liver [02377 ];
Placenta [02377 ];
Red blood cell [02377 ];
Blood [02377 ];

Tissue specificity

The long isoform is found in lymphoblasts, granulocytes and sperm [P11413 ];

Disease relevance

Disease

Defects in G6PD are the cause of chronic non-spherocytic hemolytic anemia (CNSHA) [MIM:305900]. Deficiency of G6PD is associated with hemolytic anemia in two different situations. First, in areas in which malaria has been endemic, G6PD-deficiency alleles have reached high frequencies (1% to 50%) and deficient individuals, though essentially asymptomatic in the steady state, have a high risk of acute hemolytic attacks. Secondly, sporadic cases of G6PD deficiency occur at a very low frequencies, and they usually present a more severe phenotype. Several types of CNSHA are recognized. Class-I variants are associated with severe NSHA;
class-II have an activity <10% of normal;
class-III have an activity of 10% to 60% of normal;
class-IV have near normal activity [P11413 ];

Links

SwissProt

A2IBT6; A8K8D9; P11413; Q0PHS1; Q2Q9B7; Q2Q9H2; Q2VF42

Entrez Gene

2539

HPRD

02377



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min