Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

2.4.2.14

Official Name

amidophosphoribosyltransferase

Name from literature

phosphoribosyl pyrophosphate amidotransferase

Pathway from literature

the purine de novo pathway

Pathway from KEGG

Amino Acid Metabolism; Glutamate metabolism; map00251

Nucleotide Metabolism; Purine metabolism; map00230

Organisms

Human (9606)

Genome localization

4q12[5471 ],

Rate-limiting Description

"The reciprocal plots for 5-phosphoribosyl-1-pyrophosphate (PRPP) of liver and hepatoma enzymes gave apparent KmS of 2 microM for adenine phosphoribosyltransferase and 4 microM for HGPRT, showing two orders of magnitude higher affinities for PRPP than that of the rate-limiting enzyme of de novo purine synthesis, amidophosphoribosyltransferase (EC 2.4.2.14) (Km = 400 to 900 microM)." (6327016)

Regulatory Information

Upstream transcription factor

6667;4899

Regulatory type

Detail

regulatory enzyme;

"De novo synthesis of purines at the regulatory enzyme sites of PRPP amidotransferase and IMP dehydrogenase were the major targets of the agent." (7756677)

transcriptional factor;"NRF-1(4899),Sp1(6667)"

"The human GPAT and AIRC genes are divergently transcribed from a 558 bp intergenic promoter region. Cis-acting sites and transcription factors important for bidirectional expression were identified. A cluster of sites between nt 215 and 260 are essential, although not sufficient, for expression of both genes. Two proteins from HepG2 cell nuclear extract, identified as NRF-1 and Sp1, bound to the promoter at sites within the 215-260 region." (9108165)

Gene ontology

Gene ontology

GO:0000287 (F) magnesium ion binding [Q06203 ];
GO:0006164 (P) purine nucleotide biosynthetic process [Q06203 ];
GO:0051539 (F) 4 iron, 4 sulfur cluster binding [Q06203 ];
GO:0005506 (F) iron ion binding [Q06203 ];
GO:0006541 (P) glutamine metabolic process [Q06203 ];
GO:0004044 (F) amidophosphoribosyltransferase activity [Q06203 ];
GO:0009116 (P) nucleoside metabolic process [Q06203 ];
GO:0009113 (P) purine base biosynthetic process [Q06203 ];

Tissue expression

Tissue From HPRD

Keratinocyte [01402 ];

Tissue specificity

Ubiquitously expressed [Q06203 ];

Links

SwissProt

Q06203

Entrez Gene

5471

HPRD

01402



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  Last Modified: 2009-03-24  
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