Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

1.14.13.39

Official Name

nitric-oxide synthase

Name from literature

nitric oxide synthase

Pathway from literature

nitric oxide(NO) synthesis

Pathway from KEGG

Amino Acid Metabolism; Arginine and proline metabolism; map00330

Environmental Information Processing; Signal Transduction; Calcium signaling pathway; map04020

Cellular Processes; Nervous System; Long-term depression; map04730

Human Diseases; Cancers; Small cell lung cancer; map05222

Organisms

Rat (10116)

Genome localization

12q16[24598 ], 10q25[24599 ], 4q11[24600 ],

Comments

The enzyme in brain, but not that induced in lung or liver by endotoxin, requires Ca2+. The stoichiometry is not clear, but may involve a two-electron and a one-electron oxidation step.

Rate-limiting Description

"eNOS exists in both monomeric and dimeric conformations, with the dimeric form catalyzes the rate-limiting step in the synthesis of nitric oxide, while the monomeric form catalyzes the synthesis of superoxide (O2)." (19019231)

Regulatory Information

Upstream transcription factor

25735;24253;81736;29382

Regulatory type

Detail

phosphorylation;

P29476

phosphorylation;

Q06518

phosphorylation;

Q62600

phosphorylation;

Q62600:from_uniprot:1174_Phosphothreonine

phosphorylation;

Q62600:from_uniprot:1176_Phosphoserine

phosphorylation;

Q62600:from_uniprot:494_Phosphothreonine

phosphorylation;

Q62600:from_uniprot:632_Phosphoserine

phosphorylation;

Q62600:from_uniprot:80_Phosphotyrosine

transcriptional factor;HNF-4alpha(25735)

"A unique pattern of phosphorylation determines HNF-4alpha activity as a trans-activator of IL-1beta-mediated hepatocyte iNOS expression in the presence of oxidative stress." (12466152)

transcriptional factor;CEBP(24253)

"Roles for glucose-6-phosphate dehydrogenase and CCAAT/enhancing binding protein in the regulation of inducible nitric oxide synthase gene expression in glial cells." (12930785)

transcriptional factor;NF-kappaB(81736)

"transcription factors, NF-kappaB, and iNOS have roles in ethylene glycol-induced crystal depositions in renal tubules" (17941794)

transcriptional factor;NF-kappaB(81736)

"eNOS-derived NO, in part via activation of NF-kappaB, regulates iNOS-induction by LPS" (15583003)

phosphorylation;

"phosphorylation at ser847 by CaM-KII in the hippocampus of rat brain after transient forebrain ischemia" (12218415)

phosphorylation;

"neuronal nitric-oxide synthase activity through serine 741 phosphorylation is inhibited by Calcium/calmodulin-dependent protein kinase I" (15251453)

phosphorylation;

"Akt-dependent phosphorylation of endothelial nitric-oxide synthase mediates penile erection" (11904450)

phosphorylation;

"Diminished NO/cGMP signaling in mesenteric arteries from DOCA-salt rats is caused by reduced phosphorylation of NOS3 at serine 1179 and serine 635." (14993198)

phosphorylation;

"important role for Src-mediated phosphorylation of eNOS at Tyr83 in agonist stimulation of eNOS activation and vascular relaxation" (18096817)

transcriptional factor;Ctbp1(29382)

"Binding of neuronal nitric-oxide synthase (nNOS) to carboxyl-terminal-binding protein (CtBP) changes the localization of CtBP from the nucleus to the cytosol: a novel function for targeting by the PDZ domain of nNOS." (11590170)

Gene ontology

Gene ontology

GO:0042742 (P) defense response to bacterium [Q06518 ];
GO:0008270 (F) zinc ion binding [Q06518, Q62600 ];
GO:0050660 (F) FAD binding [Q06518, Q62600, P29476 ];
GO:0016597 (F) amino acid binding [P29476 ];
GO:0020037 (F) heme binding [Q06518, Q62600, P29476 ];
GO:0005794 (C) Golgi apparatus [Q62600 ];
GO:0005509 (F) calcium ion binding [Q62600 ];
GO:0006527 (P) arginine catabolic process [P29476 ];
GO:0009055 (F) electron carrier activity [Q06518, Q62600, P29476 ];
GO:0006809 (P) nitric oxide biosynthetic process [Q06518, Q62600, P29476 ];
GO:0001917 (C) photoreceptor inner segment [P29476 ];
GO:0050661 (F) NADP binding [Q06518, Q62600, P29476 ];
GO:0042995 (C) cell projection [P29476 ];
GO:0046870 (F) cadmium ion binding [P29476 ];
GO:0001666 (P) response to hypoxia [P29476 ];
GO:0005739 (C) mitochondrion [P29476 ];
GO:0004517 (F) nitric-oxide synthase activity [Q06518, Q62600, P29476 ];
GO:0005506 (F) iron ion binding [Q06518, Q62600, P29476 ];
GO:0042383 (C) sarcolemma [P29476 ];
GO:0006801 (P) superoxide metabolic process [Q06518 ];
GO:0048471 (C) perinuclear region of cytoplasm [P29476 ];
GO:0005634 (C) nucleus [Q06518, Q62600 ];
GO:0005856 (C) cytoskeleton [Q62600 ];
GO:0005516 (F) calmodulin binding [Q06518, Q62600, P29476 ];
GO:0055114 (P) oxidation reduction [Q06518, Q62600, P29476 ];
GO:0010181 (F) FMN binding [Q06518, Q62600, P29476 ];

Subcellular localization

Localization

cell membrane [P29476 ];

golgi apparatus [Q62600 ];

myofibril [P29476 ];

cytoskeleton [Q62600 ];

membrane [Q62600 ];

cytoplasm [Q62600 ];

Links

SwissProt

P29476; Q06518; Q62600

Entrez Gene

24598; 24599; 24600



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min