Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

1.14.16.4

Official Name

tryptophan 5-monooxygenase

Name from literature

Tryptophan hydroxylase

Pathway from literature

the biosynthesis of serotonin (5-HT)/ melatonin biosynthesis

Pathway from KEGG

Amino Acid Metabolism; Tryptophan metabolism; map00380

Organisms

Mouse (10090)

Genome localization

10 D2[216343 ], 7 B4|7 23.5 cM[21990 ],

Comments

The active centre contains mononuclear iron(II). The enzyme is activated by phosphorylation, catalysed by a Ca2+-activated protein kinase. The 4a-hydroxytetrahydrobiopterin formed can dehydrate to 6,7-dihydrobiopterin, both spontaneously and by the action of EC 4.2.1.96, 4a-hydroxytetrahydrobiopterin dehydratase. The 6,7-dihydrobiopterin can be enzymically reduced back to tetrahydrobiopterin, by EC 1.5.1.34 (6,7-dihydropteridine reductase), or slowly rearranges into the more stable compound 7,8-dihydrobiopterin.

Rate-limiting Description

"We used the microdialysis technique to compare basal extracellular serotonin (5-HT) and the response to citalopram in different strains of mice with functionally different allelic forms of tryptophan hydroxylase-2 (TPH-2), the rate-limiting enzyme in brain 5-HT synthesis." (17666043)

"Tryptophan hydroxylase 2 (TPH2) is the rate limiting enzyme of serotonin synthesis in the brain." (18082956)

"Serotonin ." (5-hydroxytryptamine (5-HT) biosynthesis depends on two rate-limiting tryptophan hydroxylases (Tph): Tph1, which is expressed in peripheral organs, and Tph2, which is expressed in neurons)

"Tryptophan hydroxylase-2 (TPH2) is the rate-limiting enzyme in raphe serotonin biosynthesis, and polymorphisms of TPH2 are implicated in vulnerability to psychiatric disorders." (17613521)

"Tryptophan hydroxylase (TPH) is the initial and rate-limiting enzyme in the biosynthesis of serotonin." (17727633)

Regulatory Information

Regulatory type

Detail

phosphorylation;

P17532

phosphorylation;

Q8CGV2

phosphorylation;

P17532:from_uniprot:61_Phosphoserine; by PKA

phosphorylation;

Q8CGV2:from_uniprot:380_Phosphoserine

phosphorylation;

Q8CGV2:from_uniprot:390_Phosphoserine

Gene ontology

Gene ontology

GO:0016597 (F) amino acid binding [Q8CGV2, P17532 ];
GO:0004510 (F) tryptophan 5-monooxygenase activity [Q8CGV2, P17532 ];
GO:0055114 (P) oxidation reduction [Q8CGV2, P17532 ];
GO:0006587 (P) serotonin biosynthetic process from tryptophan [Q8CGV2, P17532 ];
GO:0005506 (F) iron ion binding [Q8CGV2, P17532 ];

Links

SwissProt

P17532; Q8CGV2

Entrez Gene

216343; 21990



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  Last Modified: 2009-03-24  
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