Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

6.3.5.5

Official Name

carbamoyl-phosphate synthase (glutamine-hydrolysing)

Name from literature

carbamoyl phosphate synthase

Pathway from literature

the de novo UTP biosynthetic pathway

Pathway from KEGG

Amino Acid Metabolism; Glutamate metabolism; map00251

Nucleotide Metabolism; Pyrimidine metabolism; map00240

Organisms

Mouse (10090)

Genome localization

5 B1[69719 ],

Comments

The product carbamoyl phosphate is an intermediate in the biosynthesis of arginine and the pyrimidine nucleotides [4]. The enzyme from Escherichia coli has three separate active sites, which are connected by a molecular tunnel that is almost 100 A in length [8]. The amidotransferase domain within the small subunit of the enzyme hydrolyses glutamine to ammonia via a thioester intermediate. The ammonia migrates through the interior of the protein, where it reacts with carboxy phosphate to produce the carbamate intermediate. The carboxy-phosphate intermediate is formed by the phosphorylation of bicarbonate by ATP at a site contained within the N-terminal half of the large subunit. The carbamate intermediate is transported through the interior of the protein to a second site within the C-terminal half of the large subunit, where it is phosphorylated by another ATP to yield the final product, carbamoyl phosphate [6].

Rate-limiting Description

"Appearance of CAD activity, the rate-limiting enzyme for pyrimidine biosynthesis, as B cells progress into and through the G1 stage of the cell cycle." (7916269)

Regulatory Information

Upstream transcription factor

17869

Regulatory type

Detail

transcriptional factor;Myc(17869)

"PML interacts with Myc, and Myc target gene expression is altered in PML-null fibroblasts." (15735755)

Links

Entrez Gene

69719



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min