Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

3.1.1.79

Official Name

hormone-sensitive lipase

Name from literature

hormone-sensitive lipase

Pathway from literature

cholesteryl ester and diacylglycerol hydrolysis/lipolysis/intracellular TG hydrolysis

Pathway from KEGG

Cellular Processes; Endocrine System; Insulin signaling pathway; map04910

Organisms

Rat (10116)

Genome localization

1q21[25330 ],

Comments

This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters [5], retinyl esters [6] and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond [8]. The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.

Rate-limiting Description

"OBJECTIVE: The hormone-sensitive lipase (HSL) is the rate-limiting enzyme in adipose tissue lipolysis." (10832769)

"The rate-limiting step in adipose tissue lipolysis is catalysed by the enzyme hormone-sensitive lipase (HSL)." (11691879)

Regulatory Information

Regulatory type

Detail

phosphorylation;

P15304

phosphorylation;

P15304:from_uniprot:195_Phosphoserine

phosphorylation;

P15304:from_uniprot:863_Phosphoserine; by PKA

phosphorylation;

P15304:from_uniprot:865_Phosphoserine

phosphorylation;

P15304:from_uniprot:959_Phosphoserine; by PKA

phosphorylation;

P15304:from_uniprot:960_Phosphoserine; by PKA

phosphorylation;

"hormone sensitive lipase is phosphorylated by 5' AMP-activated protein kinase at Ser(565) during muscle contractions, but this phosphorylation is not directly responsible for the contraction-induced activation of HSL" (15033481)

Gene ontology

Gene ontology

GO:0016298 (F) lipase activity [P15304 ];
GO:0003716 (F) RNA polymerase I transcription termination ... [P15304 ];
GO:0042134 (F) rRNA primary transcript binding [P15304 ];
GO:0033878 (F) hormone-sensitive lipase activity [P15304 ];
GO:0008203 (P) cholesterol metabolic process [P15304 ];
GO:0005901 (C) caveola [P15304 ];
GO:0005739 (C) mitochondrion [P15304 ];
GO:0005829 (C) cytosol [P15304 ];
GO:0005811 (C) lipid particle [P15304 ];
GO:0006361 (P) transcription initiation from RNA polymeras... [P15304 ];
GO:0005634 (C) nucleus [P15304 ];
GO:0016042 (P) lipid catabolic process [P15304 ];
GO:0005515 (F) protein binding [P15304 ];

Subcellular localization

Localization

cell membrane [P15304 ];

cytoplasm [P15304 ];

Links

SwissProt

P15304

Entrez Gene

25330



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  Last Modified: 2009-03-24  
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