Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

3.1.1.79

Official Name

hormone-sensitive lipase

Name from literature

hormone-sensitive lipase

Pathway from literature

cholesteryl ester and diacylglycerol hydrolysis/lipolysis/intracellular TG hydrolysis

Pathway from KEGG

Cellular Processes; Endocrine System; Insulin signaling pathway; map04910

Organisms

Mouse (10090)

Genome localization

7 A3|7 5.5 cM[16890 ],

Comments

This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters [5], retinyl esters [6] and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond [8]. The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.

Rate-limiting Description

"Translocation of hormone-sensitive lipase, the rate-limiting enzyme of lipolysis, to the lipid droplet seems to be an important step during lipolytic activation." (11246809)

Regulatory Information

Upstream transcription factor

20181;19016

Regulatory type

Detail

phosphorylation;

P54310

phosphorylation;

P54310:from_uniprot:557_Phosphoserine

phosphorylation;

P54310:from_uniprot:559_Phosphoserine

phosphorylation;

P54310:from_uniprot:650_Phosphoserine

phosphorylation;

P54310:from_uniprot:651_Phosphoserine

transcriptional factor;"PPARgamma(19016),RXRalpha(20181)"

"These results indicate that the HSL gene is transcriptionally regulated by PPARgamma/RXRalpha heterodimer, and suggest that a cis-acting element regulates the HSL gene expression." (17134676)

phosphorylation;

"perilipin expression and phosphorylation state are critical regulators of lipid storage and hydrolysis in ACS1/FATP1 cells" (11751901)

phosphorylation;

"hormone-sensitive lipase translocation requires the phosphorylation of both HSL and perilipin" (12832420)

Gene ontology

Gene ontology

GO:0003716 (F) RNA polymerase I transcription termination ... [P54310 ];
GO:0042134 (F) rRNA primary transcript binding [P54310 ];
GO:0033878 (F) hormone-sensitive lipase activity [P54310 ];
GO:0008203 (P) cholesterol metabolic process [P54310 ];
GO:0005901 (C) caveola [P54310 ];
GO:0005739 (C) mitochondrion [P54310 ];
GO:0019433 (P) triacylglycerol catabolic process [P54310 ];
GO:0005829 (C) cytosol [P54310 ];
GO:0006361 (P) transcription initiation from RNA polymeras... [P54310 ];
GO:0046340 (P) diacylglycerol catabolic process [P54310 ];
GO:0004806 (F) triacylglycerol lipase activity [P54310 ];
GO:0005634 (C) nucleus [P54310 ];
GO:0005515 (F) protein binding [P54310 ];

Subcellular localization

Localization

cell membrane [P54310 ];

cytoplasm [P54310 ];

Links

SwissProt

P54310

Entrez Gene

16890



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min