Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

3.1.1.4

Official Name

phospholipase A2

Name from literature

phospholipase a2

Pathway from literature

eicosanoid synthesis/arachidonic acid release and subsequent eicosanoid formation/prostaglandin synthesis

Pathway from KEGG

Cellular Processes; Immune System; Fc epsilon RI signaling pathway; map04664

Lipid Metabolism; Ether lipid metabolism; map00565

Lipid Metabolism; Linoleic acid metabolism; map00591

Lipid Metabolism; Glycerophospholipid metabolism; map00564

Lipid Metabolism; Arachidonic acid metabolism; map00590

Lipid Metabolism; alpha-Linolenic acid metabolism; map00592

Environmental Information Processing; Signal Transduction; MAPK signaling pathway; map04010

Environmental Information Processing; Signal Transduction; VEGF signaling pathway; map04370

Cellular Processes; Nervous System; Long-term depression; map04730

Cellular Processes; Endocrine System; GnRH signaling pathway; map04912

Organisms

Rat (10116)

Genome localization

5q36[29692 ], 7q34[360426 ], 13q21[24653 ], 12q16[29526 ], 5q36[29387 ], 2q43[362039 ], 5q36[29354 ], 10q11[29359 ],

Comments

Also acts on phosphatidylethanolamine, choline plasmalogen and phosphatides, removing the fatty acid attached to the 2-position. Requires Ca2+.

Rate-limiting Description

"In view of the potential role of prostaglandins (PGs) in development of glomerular hyperfiltration leading to diabetic nephropathy, we studied the temporal relationship of the activity of cytosolic phospholipase A2 (cPLA2), a rate-limiting enzyme for eicosanoid biosynthesis, with hyperfiltration and the histological changes in glomeruli using OLETF rats, a model for non-insulin-dependent diabetes mellitus (NIDDM)." (10482042)

Regulatory Information

Upstream transcription factor

25664;25747;24252;81736

Regulatory type

Detail

phosphorylation;

P50393

phosphorylation;

P50393:from_uniprot:268_Phosphothreonine

phosphorylation;

P50393:from_uniprot:434_Phosphoserine

phosphorylation;

P50393:from_uniprot:435_Phosphoserine

phosphorylation;

P50393:from_uniprot:437_Phosphoserine

phosphorylation;

P50393:from_uniprot:505_Phosphoserine; by MAPK

phosphorylation;

P50393:from_uniprot:535_Phosphotyrosine

phosphorylation;

P50393:from_uniprot:727_Phosphoserine

phosphorylation;

P50393:from_uniprot:729_Phosphoserine

phosphorylation;

P97570:from_uniprot:532_Phosphothreonine

transcriptional factor;"PPARalpha(25747),PPARgamma(25664),RXR"

"The regulation of sPLA2-IIA gene transcription by PPARalpha/RXR and PPARgamma/RXR heterodimers requires an interaction with a PPAR response element (PPRE) of the sPLA2-IIA promoter." (17908795)

phosphorylation;

"Administration of a daily subconvulsive dose of NMDA to rats for 21 days decreased frontal cortex NMDA receptor (NR)-1 and NR-3A subunits and increased cPLA(2) activity, phosphorylation, protein, and mRNA levels." (17550430)

phosphorylation;

"the role of cPLA(2) in MAPK phosphorylation and apoptosis in simulated ischaemia/reperfusion (SI/R)-induced injury in neonatal cardiomyocytes." (17643276)

phosphorylation;

"Basal iPLA2 activity, through the generation of 5-LO metabolites, regulates AMPA receptor phosphorylation of GluR1 subunits, an effect that might selectively influence the number of membrane receptors in area CA1 of the hippocampus." (15630695)

transcriptional factor;"CCAAT/enhancer binding protein (24252),nuclear factor-kappaB(81736),Ets"

"Transcriptional regulation of the rat type IIA phospholipase A2 gene by cAMP and interleukin-1beta in vascular smooth muscle cells: interplay of the CCAAT/enhancer binding protein (C/EBP), nuclear factor-kappaB and Ets transcription factors." (12188923)

Gene ontology

Gene ontology

GO:0032869 (P) cellular response to insulin stimulus [P04055 ];
GO:0016020 (C) membrane [P14423 ];
GO:0043498 (F) cell surface binding [P04055 ];
GO:0005737 (C) cytoplasm [P97570 ];
GO:0005509 (F) calcium ion binding [Q9QZT3, P51433, P04055, P50393, P39878, P14423 ];
GO:0016021 (C) integral to membrane [O54728 ];
GO:0045740 (P) positive regulation of DNA replication [P04055 ];
GO:0006644 (P) phospholipid metabolic process [Q9QZT3, P51433, P39878, P14423 ];
GO:0004622 (F) lysophospholipase activity [O54728, P50393 ];
GO:0016324 (C) apical plasma membrane [O54728 ];
GO:0004623 (F) phospholipase A2 activity [Q9QZT3, P51433, P97570, O54728, P50393, P39878, P14423 ];
GO:0031410 (C) cytoplasmic vesicle [P50393 ];
GO:0006633 (P) fatty acid biosynthetic process [P04055 ];
GO:0005102 (F) receptor binding [P04055 ];
GO:0005576 (C) extracellular region [Q9QZT3, P51433, P39878 ];
GO:0009395 (P) phospholipid catabolic process [P50393 ];
GO:0016042 (P) lipid catabolic process [Q9QZT3, P51433, P97570, O54728, P39878, P14423 ];
GO:0015758 (P) glucose transport [P04055 ];

Subcellular localization

Localization

extracellular [Q9QZT3, P51433, P04055, P39878 ];

cell membrane [O54728, P14423 ];

cytoplasm [P97570, P50393 ];

Links

SwissProt

O54728; P04055; P14423; P39878; P50393; P51433; P97570; Q9QZT3

Entrez Gene

24653; 29354; 29359; 29387; 29526; 29692; 360426; 362039



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min