Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

Official Name

biotin synthase

Name from literature

biotin synthase

Pathway from literature

biotin biosynthesis

Pathway from KEGG

Metabolism of Cofactors and Vitamins; Biotin metabolism; map00780


Yeast (4932)

Genome localization

-[853203 ],


This single-turnover enzyme is a member of the 'AdoMet radical ' (radical SAM) family, all members of which produce the 5'-deoxyadenosin-5'-yl radical and methionine from AdoMet [i.e. S-adenosylmethionine, or S-(5'-deoxyadenosin-5'-yl)methionine], by the addition of an electron from an iron-sulfur centre. The enzyme has both a [2Fe-2S] and a [4Fe-4S] centre, and the latter is believed to donate the electron. Two molecules of AdoMet are converted into radicals; these activate positions 6 and 9 of dethiobiotin by abstracting a hydrogen atom from each, and thereby forming 5'-deoxyadenosine. Sulfur insertion into dethiobiotin at C-6 takes place with retention of configuration [3]. The sulfur donor has not been identified to date --- it is neither elemental sulfur nor from AdoMet, but it may be from the [2Fe-2S] centre [4].

Rate-limiting Description

"utilis biotin synthase (BIO2) gene, the gene of the rate-limiting enzyme for biotin biosynthesis, and assembled it under the control of a strong promoter." (16195795)

Regulatory Information

Upstream transcription factor


Regulatory type


transcriptional factor;OAF3(853938)

"Global landscape of protein complexes in the yeast Saccharomyces cerevisiae." (16554755)

Gene ontology

Gene ontology

GO:0051537 (F) 2 iron, 2 sulfur cluster binding [P32451 ];
GO:0009102 (P) biotin biosynthetic process [P32451 ];
GO:0005739 (C) mitochondrion [P32451 ];
GO:0004076 (F) biotin synthase activity [P32451 ];
GO:0051539 (F) 4 iron, 4 sulfur cluster binding [P32451 ];
GO:0005506 (F) iron ion binding [P32451 ];

Subcellular localization


mitochondrion [P32451 ];




Entrez Gene


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  Last Modified: 2009-03-24  
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