Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

1.1.1.41

Official Name

isocitrate dehydrogenase (NAD+)

Name from literature

NAD dependent isocitrate dehydrogenase

Pathway from literature

tricarboxylic acid cycle

Pathway from KEGG

Carbohydrate Metabolism; Citrate cycle (TCA cycle); map00020

Organisms

Yeast (4932)

Genome localization

-[855691 ], -[854303 ],

Comments

Requires Mn2+ or Mg2+ for activity. Unlike EC 1.1.1.42, isocitrate dehydrogenase (NADP+), oxalosuccinate cannot be used as a substrate. In eukaryotes, isocitrate dehydrogenase exists in two forms: an NAD+-linked enzyme found only in mitochondria and displaying allosteric properties, and a non-allosteric, NADP+-linked enzyme that is found in both mitochondria and cytoplasm [7]. The enzyme from some species can also use NADP+ but much more slowly [9].

Rate-limiting Description

"Mitochondrial NAD-dependent isocitrate dehydrogenase catalyzes a rate-limiting step in the tricarboxylic acid cycle." (8626605)

Regulatory Information

Upstream transcription factor

856918

Regulatory type

Detail

phosphorylation;

P28241:from_uniprot:105_Phosphothreonine

phosphorylation;

P28241:from_uniprot:153_Phosphothreonine

phosphorylation;

P28241:from_uniprot:349_Phosphothreonine

phosphorylation;

P28241:from_uniprot:360_Phosphoserine

interact with TF;RAD3(856918)

"Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry." (11805837)

Gene ontology

Gene ontology

GO:0030145 (F) manganese ion binding [P28834, P28241 ];
GO:0006102 (P) isocitrate metabolic process [P28834, P28241 ];
GO:0000287 (F) magnesium ion binding [P28834, P28241 ];
GO:0042645 (C) mitochondrial nucleoid [P28834 ];
GO:0004449 (F) isocitrate dehydrogenase (NAD+) activity [P28834, P28241 ];
GO:0006537 (P) glutamate biosynthetic process [P28834, P28241 ];
GO:0005962 (C) mitochondrial isocitrate dehydrogenase comp... [P28834, P28241 ];
GO:0006099 (P) tricarboxylic acid cycle [P28834, P28241 ];
GO:0055114 (P) oxidation reduction [P28834, P28241 ];
GO:0005515 (F) protein binding [P28834, P28241 ];
GO:0003723 (F) RNA binding [P28834, P28241 ];

Subcellular localization

Localization

mitochondrion [P28834, P28241 ];

Links

SwissProt

P28241; P28834

Entrez Gene

854303; 855691



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  Last Modified: 2009-03-24  
  Design by Zhao Min