Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

1.2.4.1/2.3.1.12/1.8.1.4

Official Name

dihydrolipoyllysine-residue acetyltransferase

Name from literature

pyruvate dehydrogenase complex

Pathway from literature

tricarboxylic acid cycle

Pathway from KEGG

Amino Acid Metabolism; Valine, leucine and isoleucine biosynthesis; map00290

Carbohydrate Metabolism; Pyruvate metabolism; map00620

Carbohydrate Metabolism; Citrate cycle (TCA cycle); map00020

Carbohydrate Metabolism; Glycolysis / Gluconeogenesis; map00010

Amino Acid Metabolism; Glycine, serine and threonine metabolism; map00260

Amino Acid Metabolism; Valine, leucine and isoleucine degradation; map00280

Amino Acid Metabolism; Alanine and aspartate metabolism; map00252

Carbohydrate Metabolism; Butanoate metabolism; map00650

Organisms

E.coli (83333)

Genome localization

-[944794 ], -[944854 ], -[944834 ],

Comments

A multimer (24-mer or 60-mer, depending on the source) of this enzyme forms the core of the pyruvate dehydrogenase multienzyme complex, and binds tightly both EC 1.2.4.1, pyruvate dehydrogenase (acetyl-transferring) and EC 1.8.1.4, dihydrolipoyl dehydrogenase. The lipoyl group of this enzyme is reductively acetylated by EC 1.2.4.1, and the only observed direction catalysed by EC 2.3.1.12 is that where the acetyl group is passed to coenzyme A.

Rate-limiting Description

"Both transient and steady state characteristics of the mutant are presented and show in contrast to the wild-type enzyme where a rearrangement of an enzyme-NADH-pyruvate complex is rate-limiting that in the mutant the rearrangement is much faster and hydride transfer is the first slow step." (11401572)

"We have now determined that if the reactions catalyzed by E3 are made rate-limiting, the excess E3 functions equivalently to that in the native complex.." (3516150)

Gene ontology

Gene ontology

GO:0050660 (F) FAD binding [P0A9P0 ];
GO:0000287 (F) magnesium ion binding [P0AFG8 ];
GO:0004742 (F) dihydrolipoyllysine-residue acetyltransfera... [P06959 ];
GO:0006096 (P) glycolysis [P06959, P0AFG8, P0A9P0 ];
GO:0005739 (C) mitochondrion [P0AFG8, P0A9P0 ];
GO:0005886 (C) plasma membrane [P0A9P0 ];
GO:0005829 (C) cytosol [P0AFG8, P0A9P0 ];
GO:0004148 (F) dihydrolipoyl dehydrogenase activity [P0A9P0 ];
GO:0031405 (F) lipoic acid binding [P06959 ];
GO:0009055 (F) electron carrier activity [P0A9P0 ];
GO:0004739 (F) pyruvate dehydrogenase (acetyl-transferring... [P0AFG8 ];
GO:0005515 (F) protein binding [P06959, P0AFG8, P0A9P0 ];
GO:0055114 (P) oxidation reduction [P0AFG8, P0A9P0 ];
GO:0045454 (P) cell redox homeostasis [P0A9P0 ];
GO:0045254 (C) pyruvate dehydrogenase complex [P06959 ];

Subcellular localization

Localization

cell membrane [P0A9P0 ];

cytoplasm [P0A9P0 ];

Links

SwissProt

P0AFG8; P0A9P0; P06959

Entrez Gene

944834; 944854; 944794



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min