Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

2.7.1.40

Official Name

pyruvate kinase

Name from literature

pyruvate kinase

Pathway from literature

glycolysis

Pathway from KEGG

Human Diseases; Metabolic Disorders; Type II diabetes mellitus; map04930

Cellular Processes; Endocrine System; Insulin signaling pathway; map04910

Carbohydrate Metabolism; Pyruvate metabolism; map00620

Nucleotide Metabolism; Purine metabolism; map00230

Carbohydrate Metabolism; Glycolysis / Gluconeogenesis; map00010

Energy Metabolism; Carbon fixation; map00710

Organisms

Rat (10116)

Genome localization

2q34[24651 ], 8q24[25630 ],

Comments

UTP, GTP, CTP, ITP and dATP can also act as donors. Also phosphorylates hydroxylamine and fluoride in the presence of CO2.

Rate-limiting Description

"The major rate-limiting step for glycolysis was the transport of glucose across the blood-brain barrier into the brain." (16046853)

"The increased glycolytic capacity is related to high levels of rate-limiting glycolytic enzymes, such as pyruvate kinase (EC 2.7.1.40)." (291050)

"All rate-limiting enzymes of glucose metabolism which were studied (glucose-6-phosphate dehydrogenase, total hexokinases, pyruvate kinase, fructose-1,6-bisphosphatase) revealed markedly reduced activity, only glucose-6-phosphatase activity was increased." (2846196)

Regulatory Information

Upstream transcription factor

24817;171078;60661;24577

Regulatory type

Detail

phosphorylation;

P11980

phosphorylation;

P12928

phosphorylation;

P11980:from_uniprot:105_Phosphotyrosine

phosphorylation;

P11980:from_uniprot:148_Phosphotyrosine

phosphorylation;

P11980:from_uniprot:328_Phosphothreonine

phosphorylation;

P11980:from_uniprot:37_Phosphoserine

phosphorylation;

P11980:from_uniprot:45_Phosphothreonine

phosphorylation;

P11980:from_uniprot:83_Phosphotyrosine

phosphorylation;

P12928:from_uniprot:43_Phosphoserine; by PKA

phosphorylation;

P12928:from_uniprot:556_Phosphothreonine

transcriptional factor;c-Myc(24577)

"c-Myc is required for the glucose-mediated induction of this metabolic enzyme." (12480946)

transcriptional factor;"NF1 family,HNF1alpha(24817)"

"cooperative interaction between NF1 family members and HNF1alpha plays an important role in hepatic L-PK transcription" (16204235)

transcriptional factor;"c-Myc(24577),Max(60661),ChREBP(171078)"

"Maximal glucose-induced expression of the L-PK gene in INS-1-derived 832/13 cells involves increased c-Myc abundance, recruitment of c-Myc, Max, and ChREBP to the promoter, and a glucose-stimulated increase in ChREBP transactivation." (17341548)

Gene ontology

Gene ontology

GO:0000287 (F) magnesium ion binding [Q6P7S0, P12928, P11980 ];
GO:0004743 (F) pyruvate kinase activity [Q6P7S0, Q7M034, P12928, P11980 ];
GO:0006096 (P) glycolysis [Q6P7S0, P12928, P11980 ];
GO:0030955 (F) potassium ion binding [Q6P7S0, P12928, P11980 ];

Links

SwissProt

P11980; P12928; Q6P7S0; Q7M034

Entrez Gene

24651; 25630



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min