Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

2.7.1.21

Official Name

thymidine kinase

Name from literature

thymidine kinase

Pathway from literature

synthesis of dTTP/the salvage pathway of nucleotide metabolism

Pathway from KEGG

Xenobiotics Biodegradation and Metabolism; Drug metabolism - other enzymes; map00983

Nucleotide Metabolism; Pyrimidine metabolism; map00240

Organisms

Mouse (10090)

Genome localization

8 D3[57813 ], 11 E1-E2|11 78.0 cM[21877 ],

Comments

Deoxyuridine can also act as acceptor, and dGTP can act as a donor. The deoxypyrimidine kinase complex induced by Herpes simplex virus catalyses this reaction as well as those of EC 2.7.1.114 (AMP---thymidine kinase), EC 2.7.1.118 (ADP---thymidine kinase) and EC 2.7.4.9 (dTMP-kinase).

Rate-limiting Description

"5'-AdThd is a thymidine analogue that at low concentrations (<30 micrometer) can increase thymidine kinase activity, which is the rate-limiting enzyme for activation of IdUrd." (9816259)

Regulatory Information

Upstream transcription factor

13555;242705;13557;104394;13559

Regulatory type

Detail

phosphorylation;

P04184

phosphorylation;

P04184:from_uniprot:13_Phosphoserine

transcriptional factor;E2F-containing protein complexes

"G1/S-regulated E2F-containing protein complexes bind to the mouse thymidine kinase gene promoter." (8288595)

transcriptional factor;Yi1,Yi2

"Transcription of the murine thymidine kinase gene, which is coregulated with the G1/S phase transition, is activated by changing the binding of protein complexes Yi1 and Yi2 to three upstream DNA motifs." (1565617#7811275)

transcriptional factor;E2f1(13555)

"Target gene specificity of E2F and pocket protein family members in living cells." (10913163)

transcriptional factor;E2f2(242705)

"Target gene specificity of E2F and pocket protein family members in living cells." (10913163)

transcriptional factor;E2f3(13557)

"Target gene specificity of E2F and pocket protein family members in living cells." (10913163)

transcriptional factor;E2f4(104394)

"Target gene specificity of E2F and pocket protein family members in living cells." (10913163)

transcriptional factor;E2f5(13559)

"Target gene specificity of E2F and pocket protein family members in living cells." (10913163)

Gene ontology

Gene ontology

GO:0006260 (P) DNA replication [P04184, Q9R296, Q9R088, B1AQK8 ];
GO:0016773 (F) phosphotransferase activity, alcohol group ... [Q9R088 ];
GO:0004797 (F) thymidine kinase activity [P04184, Q9R296, Q9R088, B1AQK8 ];
GO:0005743 (C) mitochondrial inner membrane [Q9R088 ];
GO:0005737 (C) cytoplasm [P04184 ];
GO:0005524 (F) ATP binding [P04184, Q9R296, Q9R088, B1AQK8 ];

Subcellular localization

Localization

mitochondrion [Q9R088 ];

cytoplasm [P04184 ];

Links

SwissProt

B1AQK8; P04184; Q9R088; Q9R296

Entrez Gene

21877; 57813



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  Last Modified: 2009-03-24  
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