Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

2.6.1.16

Official Name

glutamine---fructose-6-phosphate transaminase (isomerizing)

Name from literature

glutamine-fructose-6-phosphate amidotransferase

Pathway from literature

hexosamine synthesis

Pathway from KEGG

Carbohydrate Metabolism; Aminosugars metabolism; map00530

Amino Acid Metabolism; Glutamate metabolism; map00251

Organisms

Mouse (10090)

Genome localization

6 D1|6 35.5 cM[14583 ], 11 B1.2|11 26.0 cM[14584 ],

Comments

Although the overall reaction is that of a transferase, the mechanism involves the formation of ketimine between fructose 6-phosphate and a 6-amino group from a lysine residue at the active site, which is subsequently displaced by ammonia (transamidination).

Rate-limiting Description

"We have previously shown that overexpression of the rate-limiting enzyme for hexosamine synthesis (glutamine-fructose-6-phosphate amidotransferase) in adipose tissue of transgenic mice results in skeletal muscle insulin resistance and altered regulation of leptin and adiponectin." (15613679)

Regulatory Information

Regulatory type

Detail

phosphorylation;

P47856

phosphorylation;

Q9Z2Z9

phosphorylation;

P47856:from_uniprot:259_Phosphoserine

phosphorylation;

Q9Z2Z9:from_uniprot:245_Phosphoserine

transcriptional level;Sp1

"Reporter assays using deletion and mutant variants suggested that the Sp1 sites at positions -83 to -78 and -22 to -17 both play an important role in the basal promoter activity of the mouse GFAT2 gene. Electrophoretic mobility shift assay showed DNA-protein binding at both Sp1 sites. " (11167021#9060444)

Gene ontology

Gene ontology

GO:0016051 (P) carbohydrate biosynthetic process [Q9Z2Z9, P47856 ];
GO:0005529 (F) sugar binding [Q9Z2Z9, P47856 ];
GO:0006541 (P) glutamine metabolic process [Q9Z2Z9, P47856 ];
GO:0004360 (F) glutamine-fructose-6-phosphate transaminase... [Q9Z2Z9, P47856 ];
GO:0005737 (C) cytoplasm [Q9Z2Z9, P47856 ];

Links

SwissProt

P47856; Q9Z2Z9

Entrez Gene

14583; 14584



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  Last Modified: 2009-03-24  
  Design by Zhao Min