Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

1.14.16.4

Official Name

tryptophan 5-monooxygenase

Name from literature

Tryptophan hydroxylase

Pathway from literature

the biosynthesis of serotonin (5-HT)/ melatonin biosynthesis

Pathway from KEGG

Amino Acid Metabolism; Tryptophan metabolism; map00380

Organisms

Rat (10116)

Genome localization

7q22[317675 ], 1q22[24848 ],

Comments

The active centre contains mononuclear iron(II). The enzyme is activated by phosphorylation, catalysed by a Ca2+-activated protein kinase. The 4a-hydroxytetrahydrobiopterin formed can dehydrate to 6,7-dihydrobiopterin, both spontaneously and by the action of EC 4.2.1.96, 4a-hydroxytetrahydrobiopterin dehydratase. The 6,7-dihydrobiopterin can be enzymically reduced back to tetrahydrobiopterin, by EC 1.5.1.34 (6,7-dihydropteridine reductase), or slowly rearranges into the more stable compound 7,8-dihydrobiopterin.

Rate-limiting Description

"Tryptophan hydroxylase ." (EC 1.14.16.4; L-tryptophan, tetrahydropteridine:oxygen oxidoreductase (5-hydroxylating), the enzyme catalyzing the rate-limiting step in the biosynthesis of serotonin, was purified 79-fold from the region of the raphe nucleus of rat midbrain by sequential column chromatography and disc-gel electrophoresis)

Regulatory Information

Upstream transcription factor

25337;24790

Regulatory type

Detail

phosphorylation;

P09810

phosphorylation;

P09810:from_uniprot:58_Phosphoserine; by PKA

phosphorylation;

Q8CGU9:from_uniprot:377_Phosphoserine

phosphorylation;

Q8CGU9:from_uniprot:387_Phosphoserine

transcriptional factor;"NF-Y(25337),Sp1(24790)"

"An adjacent GC-rich cis -acting sequence cooperates with the inverted CCAAT box for full activation of basal transcription; both elements are essential for full cAMP response. In pinealocytes, NF-Y and Sp1 bind the inverted CCAAT box and GC-rich-region" (12065627)

Gene ontology

Gene ontology

GO:0016597 (F) amino acid binding [Q8CGU9, P09810 ];
GO:0004510 (F) tryptophan 5-monooxygenase activity [Q8CGU9, P09810 ];
GO:0055114 (P) oxidation reduction [Q8CGU9, P09810 ];
GO:0006587 (P) serotonin biosynthetic process from tryptophan [Q8CGU9, P09810 ];
GO:0005506 (F) iron ion binding [Q8CGU9, P09810 ];

Links

SwissProt

P09810; Q8CGU9

Entrez Gene

24848; 317675



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  Last Modified: 2009-03-24  
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