Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

2.8.2.23

Official Name

[heparan sulfate]-glucosamine 3-sulfotransferase 1

Name from literature

D-glucosaminyl 3-O-sulfotransferase-1

Pathway from literature

Pathway from KEGG

Glycan Biosynthesis and Metabolism; Glycan structures - Biosynthesis 1; map01030

Glycan Biosynthesis and Metabolism; Heparan sulfate biosynthesis; map00534

Organisms

Mouse (10090)

Genome localization

10 B1[319415 ], 5 B3|5 22.0 cM[15476 ],

Comments

This enzyme differs from the other [heparan sulfate]-glucosamine 3-sulfotransferases [EC 2.8.2.29 ([heparan sulfate]-glucosamine 3-sulfotransferase 2) and EC 2.8.2.30 ([heparan sulfate]-glucosamine 3-sulfotransferase 3)] by being the most selective for a precursor of the antithrombin-binding site. It has a minimal acceptor sequence of: -> GlcNAc6S-> GlcA-> GlcN2S*+/-6S-> IdoA2S-> GlcN2S-> , the asterisk marking the target (symbols as in 2-Carb-38) using +/- to mean the presence or absence of a substituent, and > to separate a predominant structure from a minor one. Thus Glc(N2S > NAc) means a residue of glucosamine where the N carries a sulfo group mainly but occasionally an acetyl group.) [1-4]. It can also modify other precursor sequences within heparan sulfate but this action does not create functional antithrombin-binding sites. These precursors are variants of the consensus sequence: -> Glc(N2S > NAc)+/-6S-> GlcA-> GlcN2S*+/-6S-> GlcA > IdoA+/-2S-> Glc(N2S/NAc)+/-6S-> [5]. If the heparan sulfate substrate lacks 2-O-sulfation of GlcA residues, then enzyme specificity is expanded to modify selected glucosamine residues preceded by IdoA as well as GlcA [6].

Rate-limiting Description

"Endothelial cell production of anticoagulant heparan sulfate (HS(act)) is controlled by the Hs3st1 gene, which encodes the rate-limiting enzyme heparan sulfate 3-O-sulfotransferase-1 (3-OST-1)." (12671048)

Gene ontology

Gene ontology

GO:0015015 (P) heparan sulfate proteoglycan biosynthetic p... [Q8BSL4 ];
GO:0046596 (P) regulation of virion penetration into host ... [Q8BSL4 ];
GO:0016021 (C) integral to membrane [Q8BSL4, Q8BKN6, Q5GFD5 ];
GO:0008467 (F) [heparan sulfate]-glucosamine 3-sulfotransf... [Q8BSL4, Q8BKN6, Q5GFD5, O35310 ];
GO:0005515 (F) protein binding [Q8BSL4 ];
GO:0000139 (C) Golgi membrane [Q8BSL4, Q8BKN6, Q5GFD5 ];
GO:0005796 (C) Golgi lumen [O35310 ];

Subcellular localization

Localization

golgi apparatus [Q8BSL4, Q8BKN6, Q5GFD5, O35310 ];

membrane [Q8BSL4, Q8BKN6, Q5GFD5 ];

Links

SwissProt

O35310; Q5GFD5; Q8BKN6; Q8BSL4

Entrez Gene

15476; 319415



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  Last Modified: 2009-03-24  
  Design by Zhao Min