Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

Official Name

1-pyrroline-5-carboxylate dehydrogenase

Name from literature


Pathway from literature

biosynthesis of proline

Pathway from KEGG

Amino Acid Metabolism; Glutamate metabolism; map00251

Amino Acid Metabolism; Arginine and proline metabolism; map00330


Yeast (4932)

Genome localization

-[856432 ],


This enzyme can oxidize a number of 1-pyrrolines, e.g. 3-hydroxy-1-pyrroline-5-carboxylate is converted into 4-hydroxyglutamate and (R)-1-pyrroline-5-carboxylate is converted into D-glutamate. While NAD+ appears to be the better electron acceptor, NADP+ can also act, but more slowly [1,3]. In many organisms, ranging from bacteria to mammals, proline is oxidized to glutamate in a two-step process involving this enzyme and EC, proline dehydrogenase [3]. In many bacterial species, both activities are carried out by a single bifunctional enzyme [3,4].

Rate-limiting Description

"These results indicate that both enzymes, not Delta(1)-pyrroline-5-carboxylate reductase, are rate-limiting enzymes in yeast cells." (14602584)

Gene ontology

Gene ontology

GO:0010133 (P) proline catabolic process to glutamate [P07275 ];
GO:0003842 (F) 1-pyrroline-5-carboxylate dehydrogenase act... [P07275 ];
GO:0006537 (P) glutamate biosynthetic process [P07275 ];
GO:0042802 (F) identical protein binding [P07275 ];
GO:0005759 (C) mitochondrial matrix [P07275 ];
GO:0005743 (C) mitochondrial inner membrane [P07275 ];
GO:0006561 (P) proline biosynthetic process [P07275 ];
GO:0055114 (P) oxidation reduction [P07275 ];

Subcellular localization


mitochondrion [P07275 ];




Entrez Gene


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  Last Modified: 2009-03-24  
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