Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

1.2.4.1/2.3.1.12/1.8.1.4

Official Name

dihydrolipoyllysine-residue acetyltransferase

Name from literature

pyruvate dehydrogenase complex

Pathway from literature

tricarboxylic acid cycle

Pathway from KEGG

Amino Acid Metabolism; Valine, leucine and isoleucine biosynthesis; map00290

Carbohydrate Metabolism; Pyruvate metabolism; map00620

Carbohydrate Metabolism; Citrate cycle (TCA cycle); map00020

Carbohydrate Metabolism; Glycolysis / Gluconeogenesis; map00010

Amino Acid Metabolism; Glycine, serine and threonine metabolism; map00260

Amino Acid Metabolism; Valine, leucine and isoleucine degradation; map00280

Amino Acid Metabolism; Alanine and aspartate metabolism; map00252

Carbohydrate Metabolism; Butanoate metabolism; map00650

Organisms

Mouse (10090)

Genome localization

3 B[18598 ], X F3-F4|X 66.5 cM[18597 ], 9 A5.3[235339 ], 12 A3|12 15.1 cM[13382 ], 14 A1[68263 ],

Comments

A multimer (24-mer or 60-mer, depending on the source) of this enzyme forms the core of the pyruvate dehydrogenase multienzyme complex, and binds tightly both EC 1.2.4.1, pyruvate dehydrogenase (acetyl-transferring) and EC 1.8.1.4, dihydrolipoyl dehydrogenase. The lipoyl group of this enzyme is reductively acetylated by EC 1.2.4.1, and the only observed direction catalysed by EC 2.3.1.12 is that where the acetyl group is passed to coenzyme A.

Rate-limiting Description

"The pyruvate dehydrogenase enzyme complex (PDC) is rate limiting for glucose oxidation in the heart." (12663261)

"Since an increase in mitochondrial calcium levels leads to activation of the pyruvate dehydrogenase complex (the rate-limiting step for carbohydrate oxidation), the increased glucose utilization observed in isolated perfused hearts in the SERCA group may reflect a higher level of mitochondrial calcium." (17142343)

Regulatory Information

Regulatory type

Detail

phosphorylation;

P35486

phosphorylation;

P35487

phosphorylation;

Q8BMF4

phosphorylation;

Q9D051

phosphorylation;

P35486:from_uniprot:232_Phosphoserine

phosphorylation;

P35486:from_uniprot:232_Phosphoserine

phosphorylation;

P35486:from_uniprot:289_Phosphotyrosine

phosphorylation;

P35486:from_uniprot:289_Phosphotyrosine

phosphorylation;

P35486:from_uniprot:293_Phosphoserine

phosphorylation;

P35486:from_uniprot:293_Phosphoserine

phosphorylation;

P35486:from_uniprot:295_Phosphoserine

phosphorylation;

P35486:from_uniprot:295_Phosphoserine

phosphorylation;

P35486:from_uniprot:300_Phosphoserine

phosphorylation;

P35486:from_uniprot:300_Phosphoserine

phosphorylation;

P35486:from_uniprot:301_Phosphotyrosine

phosphorylation;

P35486:from_uniprot:301_Phosphotyrosine

phosphorylation;

P35487:from_uniprot:233_Phosphoserine

phosphorylation;

P35487:from_uniprot:233_Phosphoserine

phosphorylation;

P35487:from_uniprot:290_Phosphotyrosine

phosphorylation;

P35487:from_uniprot:290_Phosphotyrosine

phosphorylation;

P35487:from_uniprot:294_Phosphoserine

phosphorylation;

P35487:from_uniprot:294_Phosphoserine

phosphorylation;

P35487:from_uniprot:301_Phosphoserine

phosphorylation;

P35487:from_uniprot:301_Phosphoserine

phosphorylation;

P35487:from_uniprot:302_Phosphotyrosine

phosphorylation;

P35487:from_uniprot:302_Phosphotyrosine

phosphorylation;

Q9D051:from_uniprot:67_Phosphotyrosine

phosphorylation;

Q9D051:from_uniprot:67_Phosphotyrosine

Gene ontology

Gene ontology

GO:0050660 (F) FAD binding [Q3TIE8, O08749 ];
GO:0004742 (F) dihydrolipoyllysine-residue acetyltransfera... [Q8BMF4 ];
GO:0006096 (P) glycolysis [Q8BMF4, Q9D051, P35487, P35486 ];
GO:0005737 (C) cytoplasm [Q3TIE8 ];
GO:0004148 (F) dihydrolipoyl dehydrogenase activity [Q3TIE8, O08749 ];
GO:0031405 (F) lipoic acid binding [Q8BMF4 ];
GO:0009055 (F) electron carrier activity [Q3TIE8, O08749 ];
GO:0006086 (P) acetyl-CoA biosynthetic process from pyruvate [Q8BMF4 ];
GO:0005515 (F) protein binding [Q8BMF4 ];
GO:0045454 (P) cell redox homeostasis [Q3TIE8, O08749 ];
GO:0042391 (P) regulation of membrane potential [O08749 ];
GO:0006508 (P) proteolysis [O08749 ];
GO:0005739 (C) mitochondrion [P35487 ];
GO:0048240 (P) sperm capacitation [O08749 ];
GO:0006120 (P) mitochondrial electron transport, NADH to u... [O08749 ];
GO:0019861 (C) flagellum [O08749 ];
GO:0007369 (P) gastrulation [O08749 ];
GO:0005759 (C) mitochondrial matrix [Q9D051, O08749, P35486 ];
GO:0004739 (F) pyruvate dehydrogenase (acetyl-transferring... [Q9D051, P35487, P35486 ];
GO:0043159 (C) acrosomal matrix [O08749 ];
GO:0055114 (P) oxidation reduction [Q9D051, P35487, Q3TIE8, P35486 ];
GO:0005967 (C) mitochondrial pyruvate dehydrogenase complex [Q8BMF4 ];

Subcellular localization

Localization

mitochondrion [Q8BMF4, Q9D051, P35487, O08749, P35486 ];

Links

SwissProt

P35486; P35487; Q9D051; O08749; Q3TIE8; Q8BMF4

Entrez Gene

18597; 18598; 68263; 13382; 235339



  Copyright 2009, Center for Bioinformatics 
  Last Modified: 2009-03-24  
  Design by Zhao Min