Center for Bioinformatics
Oxidoreductases | Transferases | Hydrolases | Lyases | Isomerases | Ligases

Basic Information

Enzyme Number

2.7.1.21

Official Name

thymidine kinase

Name from literature

thymidine kinase

Pathway from literature

synthesis of dTTP/the salvage pathway of nucleotide metabolism

Pathway from KEGG

Xenobiotics Biodegradation and Metabolism; Drug metabolism - other enzymes; map00983

Nucleotide Metabolism; Pyrimidine metabolism; map00240

Organisms

Rat (10116)

Genome localization

-[24834 ],

Comments

Deoxyuridine can also act as acceptor, and dGTP can act as a donor. The deoxypyrimidine kinase complex induced by Herpes simplex virus catalyses this reaction as well as those of EC 2.7.1.114 (AMP---thymidine kinase), EC 2.7.1.118 (ADP---thymidine kinase) and EC 2.7.4.9 (dTMP-kinase).

Rate-limiting Description

"Dihydropyrimidine dehydrogenase (DPD; EC 1.3.1.2), is the rate-limiting enzyme in the pyrimidine catabolic pathway and has been shown to be the key enzyme in FdUrd catabolism." (8504424)

"METHODS/RESULTS: Incubation of ." (14C-thymidine with mouse liver extracts revealed that addition of taurocholate to the reaction medium induced significant dose-dependent inhibition in the activity of the salvage nucleotide pathway rate-limiting enzyme, thymidine kinase, while other steps of nucleotide metabolism machinery, such as the rate-limiting enzyme of de novo deoxyribonucleotide synthesis, ribonucleotide reductase and the rate-limiting enzyme of thymidine catabolism, dihydropyrimidine dehydrogenase were found to be insensitive to inhibition by taurocholate)

Regulatory Information

Upstream transcription factor

24577

Regulatory type

Detail

phosphorylation;

P27158:from_uniprot:13_Phosphoserine

transcriptional factor;c-Myc(24577)

"Cellular targets for activation by c-Myc include the DNA metabolism enzyme thymidine kinase." (9212167)

Gene ontology

Gene ontology

GO:0006260 (P) DNA replication [P27158 ];
GO:0004797 (F) thymidine kinase activity [P27158, O70599 ];
GO:0005737 (C) cytoplasm [P27158 ];
GO:0005524 (F) ATP binding [P27158 ];

Subcellular localization

Localization

cytoplasm [P27158 ];

Links

SwissProt

O70599; P27158

Entrez Gene

24834



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  Last Modified: 2009-03-24  
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